Hydrogen Effect on Organs

Hydrogen gas and hydrogen-rich water have been found to be effective in promoting brain health and function. Research has shown that hydrogen has antioxidant and anti-inflammatory effects in the brain, which can help protect against neurodegenerative diseases such as Alzheimer's and Parkinson's. Hydrogen gas has been shown to improve cognitive function in animal models of Alzheimer's disease. In one study, mice with Alzheimer's disease were exposed to hydrogen gas for one hour a day for four weeks. The results showed that the mice had improved cognitive function and reduced levels of oxidative stress in the brain. Hydrogen-rich water has also been found to have beneficial effects on the brain. In a study of healthy adults, drinking hydrogen-rich water for four weeks was found to improve cognitive function and reduce oxidative stress in the brain. In addition to its antioxidant and anti-inflammatory properties, hydrogen has also been found to have anti-apoptotic effects in the brain. Apoptosis is a process of programmed cell death that can occur in the brain as a result of injury or disease. By inhibiting apoptosis, hydrogen may help protect brain cells from damage and promote brain health. Overall, the evidence suggests that hydrogen gas and hydrogen-rich water may be effective in promoting brain health and function. While more research is needed to fully understand the mechanisms of action and potential benefits, these findings are promising and suggest that hydrogen may be a natural and safe approach to supporting brain health.

Sergej O, Darinka K, Valdemar S, Dejan J. 28-Days Hydrogen-Rich Water Supplementation Affects Exercise Capacity in Mid-Age Overweight Women: 2942 Board #225 June 1 3. 2018. Medicine and Science in Sports and Exercise. 50(5S):728-729. doi: 10.1249/01.mss.0000538402.25953.d2

Ono H, Nishijima Y, Adachi N, Sakamoto M, Kudo Y, Kaneko K, Nakao A, Imaoka T. A basic study on molecular hydrogen (H2) inhalation in acute cerebral ischemia patients for safety check with physiological parameters and measurement of blood H2 level. Med Gas Res. 2012 Aug 23;2(1):21. doi: 10.1186/2045-9912-2-21. PMID: 22916706; PMCID: PMC3457852.

Eun-Ju Y, et al. A Clinical Trial of Orally Administered Alkaline Reduced Water. Biomedical Science Letters. 2007. 13(2): 23-89. 2288-7415/(eISSN).

Seo T, Kurokawa R, Sato B. A convenient method for determining the concentration of hydrogen in water: use of methylene blue with colloidal platinum. Med Gas Res. 2012 Jan 24;2:1. doi: 10.1186/2045-9912-2-1. PMID: 22273079; PMCID: PMC3309943.

Schoenfeld MP, Ansari RR, Nakao A, Wink D. A hypothesis on biological protection from space radiation through the use of new therapeutic gases as medical counter measures. Med Gas Res. 2012 Apr 4;2:8. doi: 10.1186/2045-9912-2-8. PMID: 22475015; PMCID: PMC3348081.

Shi P, Sun W, Shi P. A hypothesis on chemical mechanism of the effect of hydrogen. Med Gas Res. 2012 Jun 21;2(1):17. doi: 10.1186/2045-9912-2-17. PMID: 22721274; PMCID: PMC3583302.

Nakayama M, Nakano H, Hamada H, Itami N, Nakazawa R, Ito S. A novel bioactive haemodialysis system using dissolved dihydrogen (H2) produced by water electrolysis: a clinical trial. Nephrol Dial Transplant. 2010 Sep;25(9):3026-33. doi: 10.1093/ndt/gfq196. Epub 2010 Apr 12. PMID: 20388631.

Liu W, Dong XS, Sun YQ, Liu Z. A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. PMID: 24955162; PMCID: PMC4057841.

Noda K, Shigemura N, Tanaka Y, Kawamura T, Hyun Lim S, Kokubo K, Billiar TR, Bermudez CA, Kobayashi H, Nakao A. A novel method of preserving cardiac grafts using a hydrogen-rich water bath. J Heart Lung Transplant. 2013 Feb;32(2):241-50. doi: 10.1016/j.healun.2012.11.004. Epub 2012 Dec 28. PMID: 23273745.

Chuai Y, Zhao L, Ni J, Sun D, Cui J, Li B, Qian L, Gao F, Cai J. A possible prevention strategy of radiation pneumonitis: combine radiotherapy with aerosol inhalation of hydrogen-rich solution. Med Sci Monit. 2011 Apr;17(4):HY1-4. doi: 10.12659/msm.881698. PMID: 21455114; PMCID: PMC3539519.

Yoritaka A, Abe T, Ohtsuka C, Maeda T, Hirayama M, Watanabe H, Saiki H, Oyama G, Fukae J, Shimo Y, Hatano T, Kawajiri S, Okuma Y, Machida Y, Miwa H, Suzuki C, Kazama A, Tomiyama M, Kihara T, Hirasawa M, Shimura H, Hattori N. A randomized double-blind multi-center trial of hydrogen water for Parkinson's disease: protocol and baseline characteristics. BMC Neurol. 2016 May 12;16:66. doi: 10.1186/s12883-016-0589-0. Erratum in: BMC Neurol. 2017 Feb 20;17 (1):35. PMID: 27176725; PMCID: PMC4865993.

Shen M, Zhang H, Yu C, Wang F, Sun X. A review of experimental studies of hydrogen as a new therapeutic agent in emergency and critical care medicine. Med Gas Res. 2014 Nov 8;4:17. doi: 10.1186/2045-9912-4-17. PMID: 25905011; PMCID: PMC4406336.

Zhang JY, Liu C, Zhou L, Qu K, Wang R, Tai MH, Lei Lei JC, Wu QF, Wang ZX. A review of hydrogen as a new medical therapy. Hepatogastroenterology. 2012 Jun;59(116):1026-32. doi: 10.5754/hge11883. PMID: 22328284.

Zhang DQ, Zhu JH, Chen WC. Acarbose: a new option in the treatment of ulcerative colitis by increasing hydrogen production. Afr J Tradit Complement Altern Med. 2012 Oct 1;10(1):166-9. PMID: 24082339; PMCID: PMC3746371.

Yuan L, Chen X, Qian L, Shen J, Cai J. Administration of hydrogen-rich saline in mice with allogeneic hematopoietic stem-cell transplantation. Med Sci Monit. 2015 Mar 12;21:749-54. doi: 10.12659/MSM.891338. PMID: 25763677; PMCID: PMC4367861.

Sanetaka S, Takeki H, Kiichiro T. Advanced research on the health benefit of reduced water. 2012. Trends in Food Science & Technology. 23(2); 124-131. doi: 10.1016/j.tifs.2011.10.009.

Tanaka Y, Saihara Y, Izumotani K, Nakamura H. Daily ingestion of alkaline electrolyzed water containing hydrogen influences human health, including gastrointestinal symptoms. Med Gas Res. 2019 Jan 9;8(4):160-166. doi: 10.4103/2045-9912.248267. PMID: 30713669; PMCID: PMC6352572.

Zhu WJ, Nakayama M, Mori T, Hao K, Terawaki H, Katoh J, Kabayama S, Ito S. Amelioration of cardio-renal injury with aging in dahl salt-sensitive rats by H2-enriched electrolyzed water. Med Gas Res. 2013 Dec 2;3(1):26. doi: 10.1186/2045-9912-3-26. PMID: 24289332; PMCID: PMC3866609.

Nakao A, Kaczorowski DJ, Wang Y, Cardinal JS, Buchholz BM, Sugimoto R, Tobita K, Lee S, Toyoda Y, Billiar TR, McCurry KR. Amelioration of rat cardiac cold ischemia/reperfusion injury with inhaled hydrogen or carbon monoxide, or both. J Heart Lung Transplant. 2010 May;29(5):544-53. doi: 10.1016/j.healun.2009.10.011. Epub 2009 Dec 24. PMID: 20036162.

Yang Y, Zhu Y, Xi X. Anti-inflammatory and antitumor action of hydrogen via reactive oxygen species. Oncol Lett. 2018 Sep;16(3):2771-2776. doi: 10.3892/ol.2018.9023. Epub 2018 Jun 26. PMID: 30127861; PMCID: PMC6096066.

Lee SH, Choi BK. Antibacterial effect of electrolyzed water on oral bacteria. J Microbiol. 2006 Aug;44(4):417-22. PMID: 16953177.

Jin D, Ryu SH, Kim HW, Yang EJ, Lim SJ, Ryang YS, Chung CH, Park SK, Lee KJ. Anti-diabetic effect of alkaline-reduced water on OLETF rats. Biosci Biotechnol Biochem. 2006 Jan;70(1):31-7. doi: 10.1271/bbb.70.31. PMID: 16428818.

Zhang Y, Sun Q, He B, Xiao J, Wang Z, Sun X. Anti-inflammatory effect of hydrogen-rich saline in a rat model of regional myocardial ischemia and reperfusion. Int J Cardiol. 2011 Apr 1;148(1):91-5. doi: 10.1016/j.ijcard.2010.08.058. Epub 2010 Sep 20. PMID: 20851484.

Gharib B, Hanna S, Abdallahi OM, Lepidi H, Gardette B, De Reggi M. Anti-inflammatory properties of molecular hydrogen: investigation on parasite-induced liver inflammation. C R Acad Sci III. 2001 Aug;324(8):719-24. doi: 10.1016/s0764-4469(01)01350-6. PMID: 11510417.

Lin CP, Chuang WC, Lu FJ, Chen CY. Anti-oxidant and anti-inflammatory effects of hydrogen-rich water alleviate ethanol-induced fatty liver in mice. World J Gastroenterol. 2017 Jul 21;23(27):4920-4934. doi: 10.3748/wjg.v23.i27.4920. PMID: 28785146; PMCID: PMC5526762.

Asada R, Kageyama K, Tanaka H, Matsui H, Kimura M, Saitoh Y, Miwa N. Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death. Oncol Rep. 2010 Dec;24(6):1463-70. doi: 10.3892/or_00001006. PMID: 21042740.

Suzuki Y, Sano M, Hayashida K, Ohsawa I, Ohta S, Fukuda K. Are the effects of alpha-glucosidase inhibitors on cardiovascular events related to elevated levels of hydrogen gas in the gastrointestinal tract? FEBS Lett. 2009 Jul 7;583(13):2157-9. doi: 10.1016/j.febslet.2009.05.052. Epub 2009 Jun 6. PMID: 19505462.

Shin MH, Park R, Nojima H, Kim HC, Kim YK, Chung JH. Atomic hydrogen surrounded by water molecules, H(H2O)m, modulates basal and UV-induced gene expressions in human skin in vivo. PLoS One. 2013 Apr 24;8(4):e61696. doi: 10.1371/journal.pone.0061696. PMID: 23637886; PMCID: PMC3634861.

Ning Y, Shang Y, Huang H, Zhang J, Dong Y, Xu W, Li Q. Attenuation of cigarette smoke-induced airway mucus production by hydrogen-rich saline in rats. PLoS One. 2013 Dec 20;8(12):e83429. doi: 10.1371/journal.pone.0083429. PMID: 24376700; PMCID: PMC3869805.

Ichihara M, Sobue S, Ito M, Ito M, Hirayama M, Ohno K. Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive review of 321 original articles. Med Gas Res. 2015 Oct 19;5:12. doi: 10.1186/s13618-015-0035-1. PMID: 26483953; PMCID: PMC4610055.

Huang Y, Xie K, Li J, Xu N, Gong G, Wang G, Yu Y, Dong H, Xiong L. Beneficial effects of hydrogen gas against spinal cord ischemia-reperfusion injury in rabbits. Brain Res. 2011 Mar 10;1378:125-36. doi: 10.1016/j.brainres.2010.12.071. Epub 2010 Dec 31. PMID: 21195696.

Matsuno N, Watanabe R, Kimura M, Iwata S, Fujiyama M, Kono S, Shigeta T, Enosawa S. Beneficial effects of hydrogen gas on porcine liver reperfusion injury with use of total vascular exclusion and active venous bypass. Transplant Proc. 2014 May;46(4):1104-6. doi: 10.1016/j.transproceed.2013.11.134. PMID: 24815139.

Zhou L, Wang X, Xue W, Xie K, Huang Y, Chen H, Gong G, Zeng Y. Beneficial effects of hydrogen-rich saline against spinal cord ischemia-reperfusion injury in rabbits. Brain Res. 2013 Jun 23;1517:150-60. doi: 10.1016/j.brainres.2013.04.007. Epub 2013 Apr 17. PMID: 23603405.

Nakayama M, Kabayama S, Nakano H, Zhu WJ, Terawaki H, Nakayama K, Katoh K, Satoh T, Ito S. Biological effects of electrolyzed water in hemodialysis. Nephron Clin Pract. 2009;112(1):c9-15. doi: 10.1159/000210569. Epub 2009 Apr 3. PMID: 19342864.

Shimouchi A, Nose K, Yamaguchi M, Ishiguro H, Kondo T. Breath hydrogen produced by ingestion of commercial hydrogen water and milk. Biomark Insights. 2009 Feb 9;4:27-32. doi: 10.4137/bmi.s2209. PMID: 19652760; PMCID: PMC2716677.

Shinbo T, Kokubo K, Sato Y, Hagiri S, Hataishi R, Hirose M, Kobayashi H. Breathing nitric oxide plus hydrogen gas reduces ischemia-reperfusion injury and nitrotyrosine production in murine heart. Am J Physiol Heart Circ Physiol. 2013 Aug 15;305(4):H542-50. doi: 10.1152/ajpheart.00844.2012. Epub 2013 Jun 14. PMID: 23771690.

Li D, Wang WC. Can hydrogen retard the progression of osteoarthritis? 2012. African J of Pharmacy and Pharmacology. 6(5); 352-354. doi: 10.5897/AJPP11.515.

Moosavi A, Bagheri F, Farkhani HR. Capabilities of hydrogen Molecules for use in the prevention and treatment in noise induced hearing loss. Sci J of Rehabilitation Med. doi: 10.22037/JRM.2013.1100134.

Jing L, Wang Y, Zhao XM, Zhao B, Han JJ, Qin SC, Sun XJ. Cardioprotective Effect of Hydrogen-rich Saline on Isoproterenol-induced Myocardial Infarction in Rats. Heart Lung Circ. 2015 Jun;24(6):602-10. doi: 10.1016/j.hlc.2014.11.018. Epub 2014 Dec 4. PMID: 25533677.

Zhao C, Yu S, Li J, Xu W, Ge R. Changes in IL-4 and IL-13 expression in allergic-rhinitis treated with hydrogen-rich saline in guinea-pig model. Allergol Immunopathol (Madr). 2017 Jul-Aug;45(4):350-355. doi: 10.1016/j.aller.2016.10.007. Epub 2017 Feb 16. PMID: 28215576.

Naito Y, Takagi T, Uchiyama K, Tomatsuri N, Matsuyama K, Fujii T, Yagi N, Yoshida N, Yoshikawa T. Chronic administration with electrolyzed alkaline water inhibits aspirin-induced gastric mucosal injury in rats through the inhibition of tumor necrosis factor-alpha expression. 2022. J of Clinical Biochemistry and Nutrition. 32; 69-81. doi: 10.3164/jcbn.32.69.

Rizkalla SW, Luo J, Kabir M, Chevalier A, Pacher N, Slama G. Chronic consumption of fresh but not heated yogurt improves breath-hydrogen status and short-chain fatty acid profiles: a controlled study in healthy men with or without lactose maldigestion. Am J Clin Nutr. 2000 Dec;72(6):1474-9. doi: 10.1093/ajcn/72.6.1474. PMID: 11101474.

Nicolson GL, Mattos GF, Settineri R, Costa C, Ellithorpe R, Rosenblatt S, Valle JL, Jimenez A, Ohta S. Clinical Effects of Hydrogen Administration: From Animal and Human Diseases to Exercise Medicine. 2016. Int J of Clinical Medicine. 7(1). doi: 10.4236/ijcm.2016.71005

Tashiro H, Kitahora T, Fujiyama Y, Baba T. Clinical evaluation of alkali-ionized water for chronic diarrhea-placebo-controlled double-blind study. 2000. J-Global. 23(2); 52-56.

Paul GH, Thomas S, Grier RM, Walter JG, Anna JH, Alexander SN. Clinical Usefulness of Hydrogen Gas as an Indicator of Left-to-Right Shunts. 1963. Circulation. 28; 542-551, doi: 10.1161/01.CIR.28.4.542.

Yonamine R, Satoh Y, Kodama M, Araki Y, Kazama T. Coadministration of hydrogen gas as part of the carrier gas mixture suppresses neuronal apoptosis and subsequent behavioral deficits caused by neonatal exposure to sevoflurane in mice. Anesthesiology. 2013 Jan;118(1):105-13. doi: 10.1097/ALN.0b013e318275146d. PMID: 23221861.

Kato S, Hokama R, Okayasu H, Saitoh Y, Iwai K, Miwa N. Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood fluidity. J Nanosci Nanotechnol. 2012 May;12(5):4019-27. doi: 10.1166/jnn.2012.6163. PMID: 22852342.

Nishimura N, Tanabe H, Adachi M, Yamamoto T, Fukushima M. Colonic hydrogen generated from fructan diffuses into the abdominal cavity and reduces adipose mRNA abundance of cytokines in rats. J Nutr. 2013 Dec;143(12):1943-9. doi: 10.3945/jn.113.183004. Epub 2013 Oct 16. PMID: 24132574.

Hong Y, Sun LI, Sun R, Chen H, Yu Y, Xie K. Combination therapy of molecular hydrogen and hyperoxia improves survival rate and organ damage in a zymosan-induced generalized inflammation model. Exp Ther Med. 2016 Jun;11(6):2590-2596. doi: 10.3892/etm.2016.3231. Epub 2016 Apr 6. PMID: 27284352; PMCID: PMC4887775.

Xie K, Fu W, Xing W, Li A, Chen H, Han H, Yu Y, Wang G. Combination therapy with molecular hydrogen and hyperoxia in a murine model of polymicrobial sepsis. Shock. 2012 Dec;38(6):656-63. doi: 10.1097/SHK.0b013e3182758646. PMID: 23160520.

Liu H, Liang X, Wang D, Zhang H, Liu L, Chen H, Li Y, Duan Q, Xie K. Combination therapy with nitric oxide and molecular hydrogen in a murine model of acute lung injury. Shock. 2015 May;43(5):504-11. doi: 10.1097/SHK.0000000000000316. PMID: 25643010.

Liu W, Shan LP, Dong XS, Liu XW, Ma T, Liu Z. Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury. World J Gastroenterol. 2013 Jan 28;19(4):492-502. doi: 10.3748/wjg.v19.i4.492. PMID: 23382627; PMCID: PMC3558572.

Oku T, Nakamura S. Comparison of digestibility and breath hydrogen gas excretion of fructo-oligosaccharide, galactosyl-sucrose, and isomalto-oligosaccharide in healthy human subjects. Eur J Clin Nutr. 2003 Sep;57(9):1150-6. doi: 10.1038/sj.ejcn.1601666. PMID: 12947435.

Lee PC, Yang YY, Huang CS, Hsieh SL, Lee KC, Hsieh YC, Lee TY, Lin HC. Concomitant inhibition of oxidative stress and angiogenesis by chronic hydrogen-rich saline and N-acetylcysteine treatments improves systemic, splanchnic and hepatic hemodynamics of cirrhotic rats. Hepatol Res. 2015 May;45(5):578-88. doi: 10.1111/hepr.12379. Epub 2014 Aug 6. PMID: 24961937.

Liu S, Liu K, Sun Q, Liu W, Xu W, Denoble P, Tao H, Sun X. Consumption of hydrogen water reduces paraquat-induced acute lung injury in rats. J Biomed Biotechnol. 2011;2011:305086. doi: 10.1155/2011/305086. Epub 2011 Jan 24. PMID: 21318114; PMCID: PMC3035012.

Xin HG, Zhang BB, Wu ZQ, Hang XF, Xu WS, Ni W, Zhang RQ, Miao XH. Consumption of hydrogen-rich water alleviates renal injury in spontaneous hypertensive rats. Mol Cell Biochem. 2014 Jul;392(1-2):117-24. doi: 10.1007/s11010-014-2024-4. Epub 2014 Mar 21. PMID: 24652103.

Ishibashi T, Sato B, Rikitake M, Seo T, Kurokawa R, Hara Y, Naritomi Y, Hara H, Nagao T. Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis: an open-label pilot study. Med Gas Res. 2012 Oct 2;2(1):27. doi: 10.1186/2045-9912-2-27. PMID: 23031079; PMCID: PMC3563451.

Sakai T, Sato B, Hara K, Hara Y, Naritomi Y, Koyanagi S, Hara H, Nagao T, Ishibashi T. Consumption of water containing over 3.5 mg of dissolved hydrogen could improve vascular endothelial function. Vasc Health Risk Manag. 2014 Oct 17;10:591-7. doi: 10.2147/VHRM.S68844. PMID: 25378931; PMCID: PMC4207582.

Kurokawa R, Seo T, Sato B, Hirano S, Sato F. Convenient methods for ingestion of molecular hydrogen: drinking, injection, and inhalation. Med Gas Res. 2015 Oct 26;5:13. doi: 10.1186/s13618-015-0034-2. PMID: 26504515; PMCID: PMC4620630.

Calloway DH, Murphy EL, Bauer D. Determination of lactose intolerance by breath analysis. Am J Dig Dis. 1969 Nov;14(11):811-5. doi: 10.1007/BF02235972. PMID: 5358883.

Tange Y, Takesawa S, Yoshitake S. Dialysate with high dissolved hydrogen facilitates dissociation of indoxyl sulfate from albumin. Nephrourol Mon. 2015 Feb 25;7(2):e26847. doi: 10.5812/numonthly.26847. PMID: 25883914; PMCID: PMC4393549.

Neale RJ. Dietary fibre and health: the role of hydrogen production. Med Hypotheses. 1988 Sep;27(1):85-7. doi: 10.1016/0306-9877(88)90091-6. PMID: 2849711.

Pshenichnyuk SA, Komolov AS. Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H2 Antioxidant Species Inside Mitochondria. J Phys Chem Lett. 2015 Apr 2;6(7):1104-10. doi: 10.1021/acs.jpclett.5b00368. Epub 2015 Mar 12. PMID: 26262957.

Nail L. A, Stanislav A. P, Ekaterina P. N, Alexander S. V, Yuri N. E, Alberto M, Alexei S. K. Dissociative electron attachment to some spinochromes: Fragment anion formation. International Journal of Mass Spectrometry. 2017. Vol. 412, Pages 26-37, ISSN 1387-3806, https://doi.org/10.1016/j.ijms.2016.12.010.

Nakayama M, Zhu WJ, Watanabe K, Gibo A, Sherif AM, Kabayama S, Ito S. Dissolved molecular hydrogen (H2) in Peritoneal Dialysis (PD) solutions preserves mesothelial cells and peritoneal membrane integrity. BMC Nephrol. 2017 Oct 31;18(1):327. doi: 10.1186/s12882-017-0741-0. PMID: 29089029; PMCID: PMC5664574.

Rheem KE, Lim YR, Lee BS, Lee SK, Seong KM, Hyun DH, Min KJ. Does alkaline-reduced hexagonal water delay the aging process in Drosophila? Geriatr Gerontol Int. 2012 Jan;12(1):151-4. doi: 10.1111/j.1447-0594.2011.00713.x. PMID: 22188498.

Ostojic SM. Does H2 Alter Mitochondrial Bioenergetics via GHS-R1α Activation? Theranostics. 2017 Mar 7;7(5):1330-1332. doi: 10.7150/thno.18745. PMID: 28435468; PMCID: PMC5399596.

Xue J, Shang G, Tanaka Y, Saihara Y, Hou L, Velasquez N, Liu W, Lu Y. Dose-dependent inhibition of gastric injury by hydrogen in alkaline electrolyzed drinking water. BMC Complement Altern Med. 2014 Mar 3;14:81. doi: 10.1186/1472-6882-14-81. PMID: 24589018; PMCID: PMC3944674.

Trivic, Tatjana, et al. "DRINKING HYDROGEN-RICH WATER FOR 4 WEEKS POSITIVELY AFFECTS SERUM ANTIOXIDANT ENZYMES IN HEALTHY MEN: A PILOT STUDY." Current Topics in Nutraceutical Research, vol. 15, no. 1, Feb. 2017, pp. 45+. Gale Academic OneFile, link.gale.com/apps/doc/A524867261/AONE?u=anon~fca5db0c&sid=googleScholar&xid=7dedf18e. Accessed 3 Apr. 2023.

Azuma T, Yamane M, Ekuni D, Kawabata Y, Kataoka K, Kasuyama K, Maruyama T, Tomofuji T, Morita M. Drinking Hydrogen-Rich Water Has Additive Effects on Non-Surgical Periodontal Treatment of Improving Periodontitis: A Pilot Study. Antioxidants (Basel). 2015 Jul 9;4(3):513-22. doi: 10.3390/antiox4030513. PMID: 26783840; PMCID: PMC4665424.

Ostojić S. Drinks with alkaline negative oxidative reduction potential improve exercise performance in physically active men and women: Double-blind, randomized, placebo-controlled, cross-over trial of efficacy and safety. 2011. Serbian Journal of Sports Sciences. ISSN 1820-6301.

Feng R, Cai M, Wang X, Zhang J, Tian Z. Early Aerobic Exercise Combined with Hydrogen-Rich Saline as Preconditioning Protects Myocardial Injury Induced by Acute Myocardial Infarction in Rats. Appl Biochem Biotechnol. 2019 Mar;187(3):663-676. doi: 10.1007/s12010-018-2841-0. Epub 2018 Jul 23. PMID: 30033489.

Ley RE, Peterson DA, Gordon JI. Ecological and evolutionary forces shaping microbial diversity in the human intestine. Cell. 2006 Feb 24;124(4):837-48. doi: 10.1016/j.cell.2006.02.017. PMID: 16497592.  

Terawaki H, Zhu WJ, Matsuyama Y, Terada T, Takahashi Y, Sakurai K, Kabayama S, Miyazaki M, Itami N, Nakazawa R, Ito S, Era S, Nakayama M. Effect of a hydrogen (H2)-enriched solution on the albumin redox of hemodialysis patients. Hemodial Int. 2014 Apr;18(2):459-66. doi: 10.1111/hdi.12112. Epub 2013 Nov 26. PMID: 24274030.  

Shimouchi A, Nose K, Takaoka M, Hayashi H, Kondo T. Effect of dietary turmeric on breath hydrogen. Dig Dis Sci. 2009 Aug;54(8):1725-9. doi: 10.1007/s10620-008-0550-1. Epub 2008 Nov 26. PMID: 19034660.  

Huang KC, Yang CC, Hsu SP, Lee KT, Liu HW, Morisawa S, Otsubo K, Chien CT. Electrolyzed-reduced water reduced hemodialysis-induced erythrocyte impairment in end-stage renal disease patients. Kidney Int. 2006 Jul;70(2):391-8. doi: 10.1038/sj.ki.5001576. Epub 2006 Jun 7. PMID: 16760903.  

Ridwan RD, Setijanto RD. Effect of electrolyzed reduced water on Wistar rats with chronic periodontitis on malondialdehyde levels. 2017. Dental Journal. 50(1). doi: 10.20473/j.djmkg.v50.i1.p-10-13.

Nagatani K, Wada K, Takeuchi S, Kobayashi H, Uozumi Y, Otani N, Fujita M, Tachibana S, Nawashiro H. Effect of hydrogen gas on the survival rate of mice following global cerebral ischemia. Shock. 2012 Jun;37(6):645-52. doi: 10.1097/SHK.0b013e31824ed57c. PMID: 22392146.  

Liang C, Liu X, Liu L, He D. [Effect of hydrogen inhalation on p38 MAPK activation in rats with lipopolysaccharide- induced acute lung injury]. Nan Fang Yi Ke Da Xue Xue Bao. 2012 Aug;32(8):1211-3. Chinese. PMID: 22931625.

Chen S, Jiang W. Effect of hydrogen injected subcutaneously on testicular tissues of rats exposed to cigarette smoke. Int J Clin Exp Med. 2015 Apr 15;8(4):5565-70. PMID: 26131139; PMCID: PMC4483942.

Fujita R, Tanaka Y, Saihara Y, Yamakita M, Ando D, Koyama K. Effect of hydrogen saturated alkaline electrolyzed water on urinary oxidative stress markers after an acute exercise: A randomized controlled trial. 2011. J of Physiological Anthropology. 30(5); 195-201. doi: 10.2114/jpa2.30.195.

Qiu XC, Jin YC, Sun Y, Luo PF, Fu JF, Chen B, Xia ZF. [Effect of hydrogen-rich saline on blood pressure and antioxidant ability of lung tissue in scalded rats following delayed resuscitation]. Zhonghua Shao Shang Za Zhi. 2010 Dec;26(6):435-8. Chinese. PMID: 21223652.

Liu Z, Cheng S, Gu C, Pei H, Hong X. Effect of Hydrogen-Rich Saline on Postoperative Intra-Abdominal Adhesion Bands Formation in Mice. Med Sci Monit. 2017 Nov 11;23:5363-5373. doi: 10.12659/msm.904669. PMID: 29127274; PMCID: PMC5695092.

Zhang J, Wu Q, Song S, Wan Y, Zhang R, Tai M, Liu C. Effect of hydrogen-rich water on acute peritonitis of rat models. Int Immunopharmacol. 2014 Jul;21(1):94-101. doi: 10.1016/j.intimp.2014.04.011. Epub 2014 Apr 29. PMID: 24793096.

Xia C, Liu W, Zeng D, Zhu L, Sun X, Sun X. Effect of hydrogen-rich water on oxidative stress, liver function, and viral load in patients with chronic hepatitis B. Clin Transl Sci. 2013 Oct;6(5):372-5. doi: 10.1111/cts.12076. Epub 2013 Jun 13. PMID: 24127924; PMCID: PMC5350887.

Nakano T, Kotani T, Imai K, Iitani Y, Ushida T, Tsuda H, Li H, Iwase A, Toyokuni S, Kikkawa F. Effect of molecular hydrogen on uterine inflammation during preterm labour. Biomed Rep. 2018 May;8(5):454-460. doi: 10.3892/br.2018.1082. Epub 2018 Mar 27. PMID: 29732148; PMCID: PMC5920961.

Matsumoto S, Ueda T, Kakizaki H. Effect of supplementation with hydrogen-rich water in patients with interstitial cystitis/painful bladder syndrome. Urology. 2013 Feb;81(2):226-30. doi: 10.1016/j.urology.2012.10.026. PMID: 23374763.

Nakao A, Toyoda Y, Sharma P, Evans M, Guthrie N. Effectiveness of hydrogen rich water on antioxidant status of subjects with potential metabolic syndrome-an open label pilot study. J Clin Biochem Nutr. 2010 Mar;46(2):140-9. doi: 10.3164/jcbn.09-100. Epub 2010 Feb 24. PMID: 20216947; PMCID: PMC2831093.

Ostojic SM, Vukomanovic B, Calleja-Gonzalez J, Hoffman JR. Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries. Postgrad Med. 2014 Sep;126(5):187-95. doi: 10.3810/pgm.2014.09.2813. PMID: 25295663.  

Shin DW, Yoon H, Kim HS, Choi YJ, Shin CM, Park YS, Kim N, Lee DH. Effects of Alkaline-Reduced Drinking Water on Irritable Bowel Syndrome with Diarrhea: A Randomized Double-Blind, Placebo-Controlled Pilot Study. Evid Based Complement Alternat Med. 2018 Apr 15;2018:9147914. doi: 10.1155/2018/9147914. PMID: 29849734; PMCID: PMC5925025.

Hiraoka A, Sasaki S, Yamada T, Shinohara A, Chiba M. Effects of drinking a water product with anti-oxidant activities in vitro on the blood levels of biomarker substances for the oxidative stress. 2006. J of Health Science. 52(6); 817-820. doi: 10.1248/jhs.52.817.

Kang KM, Kang YN, Choi IB, Gu Y, Kawamura T, Toyoda Y, Nakao A. Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors. Med Gas Res. 2011 Jun 7;1(1):11. doi: 10.1186/2045-9912-1-11. PMID: 22146004; PMCID: PMC3231938.

Shim MJ, Shin DM. Effects of H2-Rich Water Consumption on Oxidative Stress, PBMC Profiles and Their Transcriptome: A Randomized, Double-blind, Controlled Study. 2018. SNU. https://hdl.handle.net/10371/142175

Kawamura T, Gando Y, Takahashi M, Hara R, Suzuki K, Muraoka I. Effects of hydrogen bathing on exercise-induced oxidative stress and delayed-onset muscle soreness. 2016. Japanese J of Physical Fitness and Sports Med. 65(3); 297-305. doi: 10.7600/jspfsm.65.297.

He Y, Shi JZ, Zhang RJ, Zhai DX, Zhang D, Yu CQ, Liu YH. Effects of Hydrogen Gas Inhalation on Endometriosis in Rats. Reprod Sci. 2017 Feb;24(2):324-331. doi: 10.1177/1933719116655622. Epub 2016 Sep 27. PMID: 27342272.  

Da Ponte A, Giovanelli N, Nigris D, Lazzer S. Effects of hydrogen rich water on prolonged intermittent exercise. J Sports Med Phys Fitness. 2018 May;58(5):612-621. doi: 10.23736/S0022-4707.17.06883-9. Epub 2017 Apr 26. PMID: 28474871.

Tao B, Liu L, Wang N, Wang W, Jiang J, Zhang J. Effects of hydrogen-rich saline on aquaporin 1, 5 in septic rat lungs. J Surg Res. 2016 May 15;202(2):291-8. doi: 10.1016/j.jss.2016.01.009. Epub 2016 Jan 14. PMID: 27229103.

Guo SX, Fang Q, You CG, Jin YY, Wang XG, Hu XL, Han CM. Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation. J Transl Med. 2015 Jun 6;13:183. doi: 10.1186/s12967-015-0548-3. PMID: 26047940; PMCID: PMC4467622.

Yan WM, Zhang L, Chen T, Zhao GH, Long P, An J, Zhang ZM. Effects of hydrogen-rich saline on endotoxin-induced uveitis. Med Gas Res. 2017 Mar 30;7(1):9-18. doi: 10.4103/2045-9912.202905. PMID: 28480027; PMCID: PMC5402351.

Wang W, Tian L, Li Y, Wang X, Xia F, Li L, Li J, Zhang Z. Effects of hydrogen-rich saline on rats with acute carbon monoxide poisoning. J Emerg Med. 2013 Jan;44(1):107-15. doi: 10.1016/j.jemermed.2012.01.065. Epub 2012 Aug 14. PMID: 22897968.  

Zhang DQ, Feng H, Chen WC. Effects of hydrogen-rich saline on taurocholate-induced acute pancreatitis in rat. Evid Based Complement Alternat Med. 2013;2013:731932. doi: 10.1155/2013/731932. Epub 2013 Jul 28. PMID: 23983797; PMCID: PMC3745843.  

Li GM, Ji MH, Sun XJ, Zeng QT, Tian M, Fan YX, Li WY, Li N, Yang JJ. Effects of hydrogen-rich saline treatment on polymicrobial sepsis. J Surg Res. 2013 May;181(2):279-86. doi: 10.1016/j.jss.2012.06.058. Epub 2012 Jul 7. PMID: 22795273.  

Tomofuji, T., Kawabata, Y., Kasuyama, K. et al. Effects of hydrogen-rich water on aging periodontal tissues in rats. Sci Rep 4, 5534 (2014). https://doi.org/10.1038/srep05534

Takeuchi S, Mori K, Arimoto H, Fujii K, Nagatani K, Tomura S, Otani N, Osada H, Wada K. Effects of intravenous infusion of hydrogen-rich fluid combined with intra-cisternal infusion of magnesium sulfate in severe aneurysmal subarachnoid hemorrhage: study protocol for a randomized controlled trial. BMC Neurol. 2014 Sep 9;14:176. doi: 10.1186/s12883-014-0176-1. PMID: 25201463; PMCID: PMC4172868.

Nishimaki K, Asada T, Ohsawa I, Nakajima E, Ikejima C, Yokota T, Kamimura N, Ohta S. Effects of Molecular Hydrogen Assessed by an Animal Model and a Randomized Clinical Study on Mild Cognitive Impairment. Curr Alzheimer Res. 2018 Mar 14;15(5):482-492. doi: 10.2174/1567205014666171106145017. PMID: 29110615; PMCID: PMC5872374.

Higashimura Y, Baba Y, Inoue R, Takagi T, Uchiyama K, Mizushima K, Hirai Y, Ushiroda C, Tanaka Y, Naito Y. Effects of molecular hydrogen-dissolved alkaline electrolyzed water on intestinal environment in mice. Med Gas Res. 2018 Apr 18;8(1):6-11. doi: 10.4103/2045-9912.229597. PMID: 29770190; PMCID: PMC5937304.

Koyama Y, Taura K, Hatano E, Tanabe K, Yamamoto G, Nakamura K, Yamanaka K, Kitamura K, Narita M, Nagata H, Yanagida A, Iida T, Iwaisako K, Fujinawa H, Uemoto S. Effects of oral intake of hydrogen water on liver fibrogenesis in mice. Hepatol Res. 2014 Jun;44(6):663-677. doi: 10.1111/hepr.12165. Epub 2013 Jun 18. PMID: 23682614.

Guan Z, Li HF, Guo LL, Yang X. Effects of vitamin C, vitamin E, and molecular hydrogen on the placental function in trophoblast cells. Arch Gynecol Obstet. 2015 Aug;292(2):337-42. doi: 10.1007/s00404-015-3647-8. Epub 2015 Feb 14. PMID: 25681223.

Tamura T, Hayashida K, Sano M, Onuki S, Suzuki M. Efficacy of inhaled HYdrogen on neurological outcome following BRain Ischemia During post-cardiac arrest care (HYBRID II trial): study protocol for a randomized controlled trial. Trials. 2017 Oct 23;18(1):488. doi: 10.1186/s13063-017-2246-3. PMID: 29058596; PMCID: PMC5651618.  

Yoritaka A, Abe T, Ohtsuka C, Maeda T, Hirayama M, Watanabe H, Saiki H, Oyama G, Fukae J, Shimo Y, Hatano T, Kawajiri S, Okuma Y, Machida Y, Miwa H, Suzuki C, Kazama A, Tomiyama M, Kihara T, Hirasawa M, Shimura H, Hattori N. Erratum to: A randomized double-blind multi-center trial of hydrogen water for Parkinson's disease: protocol and baseline characteristics. BMC Neurol. 2017 Feb 20;17(1):35. doi: 10.1186/s12883-017-0817-2. Erratum for: BMC Neurol. 2016 May 12;16:66. PMID: 28219346; PMCID: PMC5322784.

Shimouchi A, Nose K, Shirai M, Kondo T. Estimation of molecular hydrogen consumption in the human whole body after the ingestion of hydrogen-rich water. Adv Exp Med Biol. 2012;737:245-50. doi: 10.1007/978-1-4614-1566-4_36. PMID: 22259109.

Liu C, Kurokawa R, Fujino M, Hirano S, Sato B, Li XK. Corrigendum: Estimation of the hydrogen concentration in rat tissue using an airtight tube following the administration of hydrogen via various routes. Sci Rep. 2015 Jul 20;5:9629. doi: 10.1038/srep09629. Erratum for: Sci Rep. 2014;4:5485. PMID: 26190095; PMCID: PMC4648425.

Ostojic SM. Eumelanin-driven production of molecular hydrogen: A novel element of skin defense? Med Hypotheses. 2015 Aug;85(2):237-8. doi: 10.1016/j.mehy.2015.04.014. Epub 2015 Apr 20. PMID: 25920542.  

Washabau RJ, Strombeck DR, Buffington CA, Harrold D. Evaluation of intestinal carbohydrate malabsorption in the dog by pulmonary hydrogen gas excretion. Am J Vet Res. 1986 Jun;47(6):1402-6. PMID: 3729143.

Wodick R, Lübbers DW, Grunewald W. Auswertverfahren zur Bestimmung der Organdurchblutung nach Atmung von Wasserstoffgemischen [Evaluation procedure for the determination of organ blood flow after breathing hydrogen gas mixtures]. Pflugers Arch. 1969;307(2):R51. German. PMID: 5814862.

Sone Y, Tanida S, Matsubara K, Kojima Y, Kato N, Takasu N, Tokura H. Everyday breath hydrogen excretion profile in Japanese young female students. J Physiol Anthropol Appl Human Sci. 2000 Sep;19(5):229-37. doi: 10.2114/jpa.19.229. PMID: 11155352.

Zhang DQ, Zhu JH. [Experimental studies of effects of hydrogen-rich saline in rats with severe acute pancreatitis]. Zhonghua Yi Xue Za Zhi. 2012 Sep 11;92(34):2436-40. Chinese. PMID: 23158670.

Kitamura A, Kobayashi S, Matsushita T, Fujinawa H, Murase K. Experimental verification of protective effect of hydrogen-rich water against cisplatin-induced nephrotoxicity in rats using dynamic contrast-enhanced CT. Br J Radiol. 2010 Jun;83(990):509-14. doi: 10.1259/bjr/25604811. PMID: 20505032; PMCID: PMC3473599.

Urita Y, Watanabe T, Maeda T, Arita T, Sasaki Y, Ishii T, Yamamoto T, Kugahara A, Nakayama A, Nanami M, Domon K, Ishihara S, Kato H, Hike K, Hara N, Watanabe S, Nakanishi K, Sugimoto M, Miki K. Extensive atrophic gastritis increases intraduodenal hydrogen gas. Gastroenterol Res Pract. 2008;2008:584929. doi: 10.1155/2008/584929. PMID: 18795142; PMCID: PMC2535789.

Urita Y, Watanabe T, Maeda T, Sasaki Y, Ishii T, Yamamoto T, Kugahara A, Nakayama A, Nanami M, Domon K, Ishihara S, Kato H, Hike K, Sanaka M, Nakajima H, Sugimoto M, Miki K. Extensive atrophic gastritis linked to increased levels of intraluminal hydrogen gas. Hepatogastroenterology. 2008 Sep-Oct;55(86-87):1645-8. PMID: 19102360.

Sign up for our newsletter.

Receive our deals and events.


 C-2F, Korea Bio Park, 700, Daewangpangyo-ro, 

Bundang-gu, Seongnam-si, Gyeonggi-do, Korea 13488

Tel: +82 31-628-0600

Inquiry:  biocera@biocera.co.kr

Biocera New York Office: biocera.nyc@gmail.com

Copyright © 2022 BIOCERA Co., Ltd  All rights reserved.

Sign up for our newsletter. 

Receive our deals and events for the upcoming events.

Bldg. C-2F, Korea Bio Park, 700, Daewangpangyo-ro, 

Bundang-gu, Seongnam-si, Gyeonggi-do, Korea 13488 

+82 31-628-0600 | biocera@biocera.co.kr

Biocera New York Office: biocera.nyc@biocera.co.kr 

            Facebook   |   YouTube   |   LinkedIn   |   Instagram

            Copyright © 2022   BIOCERA Co., Ltd.    All rights reserved.

DISCLAIMER: All content is provided for reference purposes only and is not intended to recommend any products as a drug or as a diagnosis for any illness or disease condition, nor to eliminate disease or other medical condition. Information and opinions are based on reputably published journals and first-hand experience. Information found and opinions expressed anywhere on our website, emails or social media channels are never to be construed as medical advice. Readers should consult appropriate health professionals on any matter relating to their health and well-being.