Hydrogen Effect on Bones

Hydrogen-rich water is a type of water that is rich in molecular hydrogen. It has been suggested that drinking hydrogen-rich water has a number of health benefits, including improving bone health. Studies have shown that hydrogen-rich water can help reduce oxidative stress in the body, which can lead to improved bone health. Oxidative stress is a condition in which there is an imbalance between the production of reactive oxygen species (ROS) and the body's ability to detoxify them. ROS can damage cells and tissues, including bone tissue, leading to bone loss and osteoporosis. Hydrogen-rich water has antioxidant properties that can help neutralize ROS and reduce oxidative stress in the body. This can help protect bone tissue from damage and promote bone health. In addition to reducing oxidative stress, hydrogen-rich water has also been shown to have anti-inflammatory properties. Inflammation is a major contributor to bone loss and osteoporosis. By reducing inflammation, hydrogen-rich water may help prevent bone loss and promote bone health. Overall, while more research is needed to fully understand the benefits of hydrogen-rich water on bones, the current evidence suggests that it may be a promising natural approach to improving bone health. Drinking hydrogen-rich water may be a simple and effective way to support bone health and prevent conditions such as osteoporosis.

Wan WL, Lin YJ, Shih PC, Bow YR, Cui Q, Chang Y, Chia WT, Sung HW. An In Situ Depot for Continuous Evolution of Gaseous H2 Mediated by a Magnesium Passivation/Activation Cycle for Treating Osteoarthritis. Angew Chem Int Ed Engl. 2018 Jul 26;57(31):9875-9879. doi: 10.1002/anie.201806159. Epub 2018 Jul 6. Erratum in: Angew Chem Int Ed Engl. 2022 May 2;61(19):e202203180. PMID: 29923670.

Xu Z, Zhou J, Cai J, Zhu Z, Sun X, Jiang C. Anti-inflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema. J Inflamm (Lond). 2012 Feb 2;9:2. doi: 10.1186/1476-9255-9-2. PMID: 22296736; PMCID: PMC3298497.

Ostojic SM, Vukomanovic B, Calleja-Gonzalez J, Hoffman JR. Effectiveness of oral and topical hydrogen for sports-related soft tissue injuries. Postgrad Med. 2014 Sep;126(5):187-95. doi: 10.3810/pgm.2014.09.2813. PMID: 25295663.

Kubota M, Shimmura S, Kubota S, Miyashita H, Kato N, Noda K, Ozawa Y, Usui T, Ishida S, Umezawa K, Kurihara T, Tsubota K. Hydrogen and N-acetyl-L-cysteine rescue oxidative stress-induced angiogenesis in a mouse corneal alkali-burn model. Invest Ophthalmol Vis Sci. 2011 Jan 21;52(1):427-33. doi: 10.1167/iovs.10-6167. PMID: 20847117.

Kawasaki H, Guan J, Tamama K. Hydrogen gas treatment prolongs replicative lifespan of bone marrow multipotential stromal cells in vitro while preserving differentiation and paracrine potentials. Biochem Biophys Res Commun. 2010 Jul 2;397(3):608-13. doi: 10.1016/j.bbrc.2010.06.009. Epub 2010 Jun 4. PMID: 20570654.

Takeuchi S, Wada K, Nagatani K, Osada H, Otani N, Nawashiro H. Hydrogen may inhibit collagen-induced platelet aggregation: an ex vivo and in vivo study. Intern Med. 2012;51(11):1309-13. doi: 10.2169/internalmedicine.51.7161. Epub 2012 Jun 1. PMID: 22687834.

Yamada T, Uchida K, Onuma K, Kuzuno J, Ujihira M, Inoue G, Sato B, Kurokawa R, Sakai R, Takaso M. Hydrogen supplementation of preservation solution improves viability of osteochondral grafts. ScientificWorldJournal. 2014;2014:109876. doi: 10.1155/2014/109876. Epub 2014 Nov 19. PMID: 25506061; PMCID: PMC4253708.

Guo JD, Li L, Shi YM, Wang HD, Hou SX. Hydrogen water consumption prevents osteopenia in ovariectomized rats. Br J Pharmacol. 2013 Mar;168(6):1412-20. doi: 10.1111/bph.12036. PMID: 23121335; PMCID: PMC3596646.

Guo J, Dong W, Jin L, Wang P, Hou Z, Zhang Y. Hydrogen-rich saline prevents bone loss in diabetic rats induced by streptozotocin. Int Orthop. 2017 Oct;41(10):2119-2128. doi: 10.1007/s00264-017-3581-4. Epub 2017 Jul 26. PMID: 28748382.

Itoh T, Hamada N, Terazawa R, Ito M, Ohno K, Ichihara M, Nozawa Y, Ito M. Molecular hydrogen inhibits lipopolysaccharide/interferon γ-induced nitric oxide production through modulation of signal transduction in macrophages. Biochem Biophys Res Commun. 2011 Jul 22;411(1):143-9. doi: 10.1016/j.bbrc.2011.06.116. Epub 2011 Jun 23. PMID: 21723254.

Hanaoka T, Kamimura N, Yokota T, Takai S, Ohta S. Molecular hydrogen protects chondrocytes from oxidative stress and indirectly alters gene expressions through reducing peroxynitrite derived from nitric oxide. Med Gas Res. 2011 Aug 4;1(1):18. doi: 10.1186/2045-9912-1-18. PMID: 22146365; PMCID: PMC3231990.

Lekic T, Manaenko A, Rolland W, Fathali N, Peterson M, Tang J, Zhang JH. Protective effect of hydrogen gas therapy after germinal matrix hemorrhage in neonatal rats. Acta Neurochir Suppl. 2011;111:237-41. doi: 10.1007/978-3-7091-0693-8_40. PMID: 21725762; PMCID: PMC3569066.

Li J, Ge Z, Fan L, Wang K. Protective effects of molecular hydrogen on steroid-induced osteonecrosis in rabbits via reducing oxidative stress and apoptosis. BMC Musculoskelet Disord. 2017 Feb 2;18(1):58. doi: 10.1186/s12891-017-1431-6. PMID: 28148301; PMCID: PMC5288900.

Sun Y, Shuang F, Chen DM, Zhou RB. Treatment of hydrogen molecule abates oxidative stress and alleviates bone loss induced by modeled microgravity in rats. Osteoporos Int. 2013 Mar;24(3):969-78. doi: 10.1007/s00198-012-2028-4. Epub 2012 May 31. PMID: 22648000.

Cai WW, Zhang MH, Yu YS, Cai JH. Treatment with hydrogen molecule alleviates TNFα-induced cell injury in osteoblast. Mol Cell Biochem. 2013 Jan;373(1-2):1-9. doi: 10.1007/s11010-012-1450-4. Epub 2012 Dec 1. PMID: 23212446.

Li DZ, Zhang QX, Dong XX, Li HD, Ma X. Treatment with hydrogen molecules prevents RANKL-induced osteoclast differentiation associated with inhibition of ROS formation and inactivation of MAPK, AKT and NF-kappa B pathways in murine RAW264.7 cells. J Bone Miner Metab. 2014 Sep;32(5):494-504. doi: 10.1007/s00774-013-0530-1. Epub 2013 Nov 7. PMID: 24196871.

Qian L, Mei K, Shen J, Cai J. Administration of hydrogen-rich saline protects mice from lethal acute graft-versus-host disease (aGVHD). Transplantation. 2013 Mar 15;95(5):658-62. doi: 10.1097/TP.0b013e31827e6b23. PMID: 23503500.

Kato S, Matsuoka D, Miwa N. Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR and 2,2'-bipyridyl methods. Mater Sci Eng C Mater Biol Appl. 2015 Aug;53:7-10. doi: 10.1016/j.msec.2015.03.064. Epub 2015 Apr 11. PMID: 26042683.

Li FY, Zhu SX, Wang ZP, Wang H, Zhao Y, Chen GP. Consumption of hydrogen-rich water protects against ferric nitrilotriacetate-induced nephrotoxicity and early tumor promotional events in rats. Food Chem Toxicol. 2013 Nov;61:248-54. doi: 10.1016/j.fct.2013.10.004. Epub 2013 Oct 16. PMID: 24140467.

Tsubone H, Hanafusa M, Endo M, Manabe N, Hiraga A, Ohmura H, Aida H. Effect of Treadmill Exercise and Hydrogen-rich Water Intake on Serum Oxidative and Anti-oxidative Metabolites in Serum of Thoroughbred Horses. J Equine Sci. 2013;24(1):1-8. doi: 10.1294/jes.24.1. Epub 2013 Apr 16. PMID: 24833996; PMCID: PMC4013981.

Settineri, R. , Ji, J. , Luo, C. , Ellithorpe, R. R. , Mattos, G. F. D. , Rosenblatt, S. , LaValle, J. , Jinenez, A. , Ohta, S. , & Nicolson, G. L. (2016). Effects of Hydrogenized Water on Intracellular Biomarkers for Antioxidants, Glucose Uptake, Insulin Signaling and SIRT 1 and Telomerase Activity. American Journal of Food and Nutrition, 4(6), 161-168.

Yanagihara T, Arai K, Miyamae K, Sato B, Shudo T, Yamada M, Aoyama M. Electrolyzed hydrogen-saturated water for drinking use elicits an antioxidative effect: a feeding test with rats. Biosci Biotechnol Biochem. 2005 Oct;69(10):1985-7. doi: 10.1271/bbb.69.1985. PMID: 16244454.

Ohsawa I, Ishikawa M, Takahashi K, Watanabe M, Nishimaki K, Yamagata K, Katsura K, Katayama Y, Asoh S, Ohta S. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med. 2007 Jun;13(6):688-94. doi: 10.1038/nm1577. Epub 2007 May 7. PMID: 17486089.

Zhang W, Huang C, Sun A, Qiao L, Zhang X, Huang J, Sun X, Yang X, Sun S. Hydrogen alleviates cellular senescence via regulation of ROS/p53/p21 pathway in bone marrow-derived mesenchymal stem cells in vivo. Biomed Pharmacother. 2018 Oct;106:1126-1134. doi: 10.1016/j.biopha.2018.07.020. Epub 2018 Jul 17. PMID: 30119179.

Sun Q, Han W, Hu H, Fan D, Li Y, Zhang Y, Lv Y, Li M, Pan S. Hydrogen alleviates hyperoxic acute lung injury related endoplasmic reticulum stress in rats through upregulation of SIRT1. Free Radic Res. 2017 Jun;51(6):622-632. doi: 10.1080/10715762.2017.1351027. Epub 2017 Jul 21. PMID: 28675985.

Yu J, Yu Q, Liu Y, Zhang R, Xue L. Hydrogen gas alleviates oxygen toxicity by reducing hydroxyl radical levels in PC12 cells. PLoS One. 2017 Mar 31;12(3):e0173645. doi: 10.1371/journal.pone.0173645. PMID: 28362819; PMCID: PMC5375132.

Han AL, Park SH, Park MS. Hydrogen Treatment Protects against Cell Death and Senescence Induced by Oxidative Damage. J Microbiol Biotechnol. 2017 Feb 28;27(2):365-371. doi: 10.4014/jmb.1608.08011. PMID: 27780950.

Yang T, Wang L, Sun R, Chen H, Zhang H, Yu Y, Wang Y, Wang G, Yu Y, Xie K. HYDROGEN-RICH MEDIUM AMELIORATES LIPOPOLYSACCHARIDE-INDUCED BARRIER DYSFUNCTION VIA RHOA-MDIA1 SIGNALING IN CACO-2 CELLS. Shock. 2016 Feb;45(2):228-37. doi: 10.1097/SHK.0000000000000503. PMID: 26529665.

Lee J, Yang G, Kim YJ, Tran QH, Choe W, Kang I, Kim SS, Ha J. Hydrogen-rich medium protects mouse embryonic fibroblasts from oxidative stress by activating LKB1-AMPK-FoxO1 signal pathway. Biochem Biophys Res Commun. 2017 Sep 23;491(3):733-739. doi: 10.1016/j.bbrc.2017.07.119. Epub 2017 Jul 22. PMID: 28743498.

Xu F, Yu S, Qin M, Mao Y, Jin L, Che N, Liu S, Ge R. Hydrogen-Rich Saline Ameliorates Allergic Rhinitis by Reversing the Imbalance of Th1/Th2 and Up-Regulation of CD4+CD25+Foxp3+Regulatory T Cells, Interleukin-10, and Membrane-Bound Transforming Growth Factor-β in Guinea Pigs. Inflammation. 2018 Feb;41(1):81-92. doi: 10.1007/s10753-017-0666-6. PMID: 28894978.

Chen Q, Chen P, Zhou S, Yan X, Zhang J, Sun X, Yuan H, Yu W. Hydrogen-rich saline attenuated neuropathic pain by reducing oxidative stress. Can J Neurol Sci. 2013 Nov;40(6):857-63. doi: 10.1017/s0317167100016024. PMID: 24257230.

Zhang YG, Sheng QS, Wang ZJ, Lv LI, Zhao W, Chen JM, Xu H. Hydrogen-rich saline promotes motor functional recovery following peripheral nerve autografting in rats. Exp Ther Med. 2015 Aug;10(2):727-732. doi: 10.3892/etm.2015.2518. Epub 2015 May 26. PMID: 26622383; PMCID: PMC4508974.

Lei W, Zi-quan L, Yi-ling H, Yao-jun G. Hydrogen-rich water inhibits mitochondrial oxidative stress and inflammation in the skeletal muscle after eccentric exercise. Chinese Journal of tissue Engineering Research. 19(29):4682-4687. 2015. doi: 10.3969/j.issn.2095-4344.2015.29.018

Hu Z, Wu B, Meng F, Zhou Z, Lu H, Zhao H. Impact of molecular hydrogen treatments on the innate immune activity and survival of zebrafish (Danio rerio) challenged with Aeromonas hydrophila. Fish Shellfish Immunol. 2017 Aug;67:554-560. doi: 10.1016/j.fsi.2017.05.066. Epub 2017 Jun 16. PMID: 28630014.

Wan WL, Lin YJ, Chen HL, Huang CC, Shih PC, Bow YR, Chia WT, Sung HW. In Situ Nanoreactor for Photosynthesizing H2 Gas To Mitigate Oxidative Stress in Tissue Inflammation. J Am Chem Soc. 2017 Sep 20;139(37):12923-12926. doi: 10.1021/jacs.7b07492. Epub 2017 Sep 11. PMID: 28870078.

Atsushi H, Hitomi I, Eri S, Kazuto K, Hikaru S, Atsuko S, Mika S, Koshi K, Yoshihiro Y. In Vitro Physicochemical Properties of Neutral Aqueous Solution Systems (Water Products as Drinks) Containing Hydrogen Gas, 2-Carboxyethyl Germanium Sesquioxide, and Platinum Nanocolloid as Additives. Journal of Health Science. 2010. 56(2) 167-174.

Ge Y, Wu F, Sun X, Xiang Z, Yang L, Huang S, Lu Z, Sun Y, Yu WF. Intrathecal infusion of hydrogen-rich normal saline attenuates neuropathic pain via inhibition of activation of spinal astrocytes and microglia in rats. PLoS One. 2014 May 23;9(5):e97436. doi: 10.1371/journal.pone.0097436. Erratum in: PLoS One. 2014;9(9):e109482. PMID: 24857932; PMCID: PMC4032255.

Kawaguchi M, Satoh Y, Otsubo Y, Kazama T. Molecular hydrogen attenuates neuropathic pain in mice. PLoS One. 2014 Jun 18;9(6):e100352. doi: 10.1371/journal.pone.0100352. PMID: 24941001; PMCID: PMC4062523.

Meng J, Yu P, Jiang H, Yuan T, Liu N, Tong J, Chen H, Bao N, Zhao J. Molecular hydrogen decelerates rheumatoid arthritis progression through inhibition of oxidative stress. Am J Transl Res. 2016 Oct 15;8(10):4472-4477. PMID: 27830032; PMCID: PMC5095341.

Ren JD, Wu XB, Jiang R, Hao DP, Liu Y. Molecular hydrogen inhibits lipopolysaccharide-triggered NLRP3 inflammasome activation in macrophages by targeting the mitochondrial reactive oxygen species. Biochim Biophys Acta. 2016 Jan;1863(1):50-5. doi: 10.1016/j.bbamcr.2015.10.012. Epub 2015 Oct 18. PMID: 26488087.

Sobue S, Inoue C, Hori F, Qiao S, Murate T, Ichihara M. Molecular hydrogen modulates gene expression via histone modification and induces the mitochondrial unfolded protein response. Biochem Biophys Res Commun. 2017 Nov 4;493(1):318-324. doi: 10.1016/j.bbrc.2017.09.024. Epub 2017 Sep 7. PMID: 28890349.

Iuchi, K., Imoto, A., Kamimura, N. et al. Molecular hydrogen regulates gene expression by modifying the free radical chain reaction-dependent generation of oxidized phospholipid mediators. Sci Rep 6, 18971 (2016). https://doi.org/10.1038/srep18971.

Kamimura N, Ichimiya H, Iuchi K, Ohta S. Molecular hydrogen stimulates the gene expression of transcriptional coactivator PGC-1α to enhance fatty acid metabolism. NPJ Aging Mech Dis. 2016 Apr 28;2:16008. doi: 10.1038/npjamd.2016.8. PMID: 28721265; PMCID: PMC5515010.

Lin, Y., Ohkawara, B., Ito, M. et al. Molecular hydrogen suppresses activated Wnt/β-catenin signaling. Sci Rep 6, 31986 (2016). https://doi.org/10.1038/srep31986 

Nishiwaki H, Ito M, Negishi S, Sobue S, Ichihara M, Ohno K. Molecular hydrogen upregulates heat shock response and collagen biosynthesis, and downregulates cell cycles: meta-analyses of gene expression profiles. Free Radic Res. 2018 Apr;52(4):434-445. doi: 10.1080/10715762.2018.1439166. Epub 2018 Mar 20. PMID: 29424253.

Ryoo KK, Lee YB, Lee JK, Lee MY, Permeability and dissolvability of cathodic electrolyzed water for electrophoretic gel and green tea components. Journal of the Korea Academia-Industrial Cooperation Society. 2005. Vol. 6 No. 1, pp 87-93.

Wang WN, Xie KL, Chen HG, Han HZ, Wang GL, Yu YH. Regulative effects of hydrogen-rich medium on monocytic adhesion and vascular endothelial permeability. Zhonghua Yi Xue Za Zhi. 2013 Nov 19;93(43):3467-9. Chinese. PMID: 24423914.

Atsushi H, Masumi T, Takahiro S, Atusok S, Momoko C, Mika Shirao, Yoshihiro Y. Studies on the properties and real existence of aqueous solution systems that are assumed to have antioxidant activities by the action of active hydrogen. Journal of Health Science. 2004. Vol. 50, No. 5, pp. 456-465.

Li J, Wu X, Chen Y, Zeng R, Zhao Y, Chang P, Wang D, Zhao Q, Deng Y, Li Y, Alam HB, Chong W. The Effects of Molecular Hydrogen and Suberoylanilide Hydroxamic Acid on Paraquat-Induced Production of Reactive Oxygen Species and TNF-α in Macrophages. Inflammation. 2016 Dec;39(6):1990-1996. doi: 10.1007/s10753-016-0434-z. PMID: 27624060.

Hanaoka K, Sun D, Lawrence R, Kamitani Y, Fernandes G. The mechanism of the enhanced antioxidant effects against superoxide anion radicals of reduced water produced by electrolysis. Biophys Chem. 2004 Jan 1;107(1):71-82. doi: 10.1016/j.bpc.2003.08.007. PMID: 14871602.

Zhao J, Zhang M, Li Y, Zhang Z, Chen M, Liu T, Zhang J, Shan A. Therapeutic Effect of Hydrogen Injected Subcutaneously on Onion Poisoned Dogs. J Vet Res. 2017 Dec 27;61(4):527-533. doi: 10.1515/jvetres-2017-0068. PMID: 29978119; PMCID: PMC5937354. Therapeutic Effect of Hydrogen Injected Subcutaneously on Onion Poisoned Dogs.

Zhao S, Mei K, Qian L, Yang Y, Liu W, Huang Y, Zhang C, Sun X, Liu C, Li B, Gao F, Cai J, Ni J. Therapeutic effects of hydrogen-rich solution on aplastic anemia in vivo. Cell Physiol Biochem. 2013;32(3):549-60. doi: 10.1159/000354459. Epub 2013 Aug 30. PMID: 24008771.  

Chen Y, Chen H, Xie K, Liu L, Li Y, Yu Y, Wang G. H2 Treatment Attenuated Pain Behavior and Cytokine Release Through the HO-1/CO Pathway in a Rat Model of Neuropathic Pain. Inflammation. 2015 Oct;38(5):1835-46. doi: 10.1007/s10753-015-0161-x. PMID: 25820467.

Sign up for our newsletter.

Receive our deals and events.


 C-2F, Korea Bio Park, 700, Daewangpangyo-ro, 

Bundang-gu, Seongnam-si, Gyeonggi-do, Korea 13488

Tel: +82 31-628-0600

Inquiry:  biocera@biocera.co.kr

Biocera New York Office: biocera.nyc@gmail.com

Copyright © 2022 BIOCERA Co., Ltd  All rights reserved.

Sign up for our newsletter. 

Receive our deals and events for the upcoming events.

[HQ] Bldg. C-2F, Korea Bio Park, 700, Daewangpangyo-ro, 

Bundang-gu, Seongnam-si, Gyeonggi-do, Korea 13488 

+82 31-628-0600 | biocera@biocera.co.kr

[NY Office] biocera.nyc@biocera.co.kr 

            Facebook   |   YouTube   |   LinkedIn   |   Instagram

DISCLAIMER: All content is provided for reference purposes only and is not intended to recommend any products as a drug or as a diagnosis for any illness or disease condition, nor to eliminate disease or other medical condition. Information and opinions are based on reputably published journals and first-hand experience. Information found and opinions expressed anywhere on our website, emails or social media channels are never to be construed as medical advice. Readers should consult appropriate health professionals on any matter relating to their health and well-being.

            Copyright © 2022   BIOCERA Co., Ltd.    All rights reserved.